The 41-year-old footballer announced his decision on November 5.
"Portugal will stay in my heart forever," Ronaldo posted on his official social media page.
The Portuguese star made his international debut on August 20, 2003, in a friendly match against Kazakhstan.
Over his 23-year international career, Ronaldo earned 234 caps and scored 146 goals for Portugal. He stands as the most-capped and highest-scoring male player in the history of international football.
During his tenure with the national team, the striker led Portugal to victory at the 2016 UEFA European Championship, as well as winning the UEFA Nations League titles in the 2018/19 and 2024/25 seasons.